On June 22, the United States Air Force and Navy attacked three Iranian nuclear sites: Fordow, Natanz, and Isfahan, in an operation called "Midnight Hammer". Seven Northrop B-2 Spirit bombers of the 509th Bomb Wing flew non-stop from Whiteman Air Force Base in Missouri.

Seven B-2 stealth bombers tasked with hitting the nuclear sites flew into Iran and avoided detection. At the same time, two dozen cruise missiles were launched at the Isfahan site from a submarine - in total, 75 "precision-guided weapons" were used during the operation.

Russia has said there was no proof Iran has ever run a nuclear weapons program. The former head of the IAEA, Mohamed ElBaradei, stated that he had seen "maybe some studies about possible weaponization", but "no evidence" of "an active weaponization program" as of October 2007.